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. 2016 Mar 2;65(5):1164–1178. doi: 10.2337/db15-1315

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© 2016 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

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RA-mediated differential expression of adipogenic genes during adipocyte differentiation of ASCs. Adipocyte differentiation was induced in SC and VS ASCs from S11 using a standard adipogenic cocktail. RNA was collected at various time points during differentiation (D0, D2, D4, and D6), and the expression of adipogenic genes was analyzed by qPCR. The representative graphs show gene expression of CEBPB (A), CEBPD (B), CEBPA (C), and PPARG (D) in SC and VS ASCs from S11 at the various time points indicated (n = 2). Adipocyte differentiation was induced in SC ASCs from S11 using a standard adipogenic cocktail, with or without RA (10 μmol/L) treatment. RNA was collected at various time points (D0, D2, D4, and D6). The representative graphs show gene expression of CEBPB (E), CEBPD (F), CEBPA (G), and PPARG (H) in SC ASCs treated with or without RA during differentiation at various time points (n = 2). *P < 0.05 denotes significance. Similar results were obtained in cells from S12.