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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Microbiol. 2016 May 6;101(2):210–223. doi: 10.1111/mmi.13384

Fig. 1.

Fig. 1

L-2,3-diaminopropionate elicits a proline requirement in S. enterica. A) Wild type S. enterica (DM14828) was grown at 37°C in glucose minimal medium containing no addition (○); 0.25 mM Dap (■); or 0.25 mM Dap and 1 mM proline (▲). The data reflect experiments performed in triplicate. Error bars representing the standard deviation are not visible because the replicates deviated less than 5 % from the mean. B) Proline biosynthesis in S. enterica occurs in four steps, beginning with the ATP-dependent phosphorylation of glutamate, generating γ-glutamyl 5-phosphate (1). The conversion of glutamate 5-semialdehyde (2) to 1-pyrroline-5-carboxylate (3) occurs spontaneously (dashed line). Proline feedback-regulates the first enzyme (ProB) in the pathway.