Table 2.
Dap inhibits ProC in vivo
| Producer strainc |
|||
|---|---|---|---|
| Reporter straina | Mediab | Wt/pDM1462 (DM15352) |
proC/pDM1462 (DM15356) |
| proAB (DM5846) | − Dap | +d | + |
| + Dap | + | − | |
| proC (TT9667) | − Dap | + | − |
| + Dap | + | − | |
a
Proline auxotrophs served as reporter strains for P5C or proline utilization. Strains were grown in rich medium then embedded in soft-agar overlaid on minimal growth medium lacking proline.
b
Growth was assessed on minimal glucose medium containing arabinose (1.3 mM). Dap was added to the growth medium at a final concentration of 0.25 mM, where indicated.
c
Producer strains were grown on NB agar. Single colonies were stabbed into agar overlays of the reporter strain.
d
Cross-feeding was designated as growth (+) or no growth (−) based on turbidity of the reporter strain after 48 hours at 37˙C. Outgrowth of the producer strains (not shown) from the sites of inoculation is described in the text.