Fig 5. Predictive dose-response confirm the results in each bioassay per farm.

Analysis were based on three different concentrations per acaricide i.e. alpha-cypermethrin at 0.002%, 0.02%, and 0.5%; amitraz at 0.002%, 0.1%, and 0.25% and ivermectin at 0.01%, 0.1%, and 0.5% for the minimum, medium or discriminatory and maximum doses, respectively. All farms except farm 8 showed resistance levels (more than 10%) according to use frequency of each acaricide.