Table 2.
Affinity of 18D1-IgG1 variants for cell expressed Fn14.
| Interaction | Number of experiments | Single KD values (nM) | Averaged KD (nM) |
|---|---|---|---|
| 18D1-IgG1 – Fn14 | 11 | 6, 9, 4, 4, 6, 4, 3, 5, 4, 7, 2 | 5 ± 2 |
| GpL(CT-HC)-18D1-IgG1 – Fn14 | 4 | 14, 20, 22, 10 | 17 ± 6a |
| GpL(CT-LC)-18D1-IgG1 – Fn14 | 4 | 9, 17, 25, 9 | 15 ± 8a |
| GpL(NT-LC)-18D1-IgG1 – Fn14 | 4 | 71, 77, 98, 50 | 74 ± 20b |
All possible pairs of antibody variants have been analyzed according to Bonferroni's test.
a
not significantly different from KD of 18D1-IgG1
b
significantly different from KD of 18D1-IgG1 (p<0.001)