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Overlaid deconvoluted mass spectra of the scFc subunit from mAb-A treated with different concentration of peracetic acid (A). To generate oxidized samples for analysis, mAb-A was incubated for 2 hr at 30°C with increasing concentrations of peracetic acid (0–500 µM). The sum of 1-ox, 2-ox and 3-ox species shown in panel A was plotted as a function of peracetic acid concentration (B).
