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. Author manuscript; available in PMC: 2017 Apr 7.
Published in final edited form as: Transplantation. 2016 Jan;100(1):217–226. doi: 10.1097/TP.0000000000000840

Figure 2. Reactivity of B cell clones’ supernatants to HLA class I native, denatured and cryptic epitopes.

Figure 2

The reactivity of non-polyreactive antibody 3D4 and polyreactive antibodies 2B4, 2D9, 4G4 and 4G10 to HLA class I antigens was tested by Luminex using regular single antigen beads (orange bars), iBEADS (blue bars) and regular single antigen beads treated with acid (green bars). Each bar represents reactivity to one corresponding class I molecule labeled below. Only the 50 antigens towards which the supernatants reacted the most are depicted for each clone. Antigens are sorted based on the supernatant reactivity to regular single antigen beads. Complete reactivity profiles are reported in supplementary Figure S1-S5.