(A) Disruption of cdk-8 or cic-1 in the hlh-3 null mutant n5469 enhances I4 misspecification.
(B) Expressing a wild-type (WT), but not a kinase-dead (KD), copy of cdk-8 cDNA using the dpy-22 promoter rescues I4 misspecification in cdk-8; hlh-3 double mutants.
(C) Overexpression of phosphomimetic CYH-1DD, but not non-phosphorylatable CYH-1AA, using the dpy-22 promoter suppresses I4 defects in cdk-8; hlh-3 mutants.
(D) Overexpression of kinase-dead CDK-7KD, but not phosphomimetic CDK-7EE, using the dpy-22 promoter rescues I4 defects in cdk-8; hlh-3 mutants. o.e., overexpression. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. See also Figures S5 and S6.