Table 2.

Percentage of apoptotic cells in AO/EB staining

Serial number	Treatment	% apoptotic cells
1	Normal control	16.54±1.06
2	DOX control, 1 µM	54.78±3.56*
3	DOX, 1 µM + RUT, 100 µM	31.22±3.76#
4	RUT, 100 µM	12.56±2.76##

Notes: Data represent mean ± SEM of three tests in triplicate for the effect of RUT on cellular apoptosis against DOX-induced neurotoxicity in differentiated IMR32 cells,

^*P<0.001 compared to normal control;

^#P<0.01 and

^##P<0.001 compared to DOX control.

Abbreviations: DOX, doxorubicin; RUT, rutin; AO/EB, acridine orange/ethidium bromide; SEM, standard error of the mean.