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. 2017 Mar 24;13(3):e1006686. doi: 10.1371/journal.pgen.1006686

Fig 1. fkbA sRNAs are present in the FK506 resistant epimutants isolates of the r3b2Δ mutant strains.

Fig 1

The numbers of the isolates correspond to those in S1 Table. With the exception noted below, the isolates analyzed here do not include those confirmed to harbor Mendelian mutations. (A) The sRNA enriched samples (35 μg) from all of the r3b2Δ FK506 resistant isolates lacking mutations in the target genes were obtained after incubation for 48 hours on MMC media supplemented with 1 μg/ml of FK506. sRNA blots were hybridized with an antisense-specific probe to detect fkbA sRNA (see Methods). 5S rRNA probe was used as a loading control. Abundant sRNAs were detected in all of the strains with the exception of isolate 7 of the MU429 strain that was confirmed to have a Mendelian mutation. (B) Signals from blots presented in panel (A) were quantified by densitometry and the results were plotted. The bars indicate the standard deviation.