Fig 5. Detection of fkbA sRNAs in the FK506 resistant epimutant isolates of rdrp3Δ mutant strains.

(A) The numbers of the isolates correspond to those in S1 Table. The isolates analyzed here do not include those confirmed to harbor Mendelian mutations. The sRNA enriched samples (35 μg) from the rdrp3Δ FK506 resistant isolates lacking mutations in the target genes were obtained after 48 hour incubation at room temperature on MMC media pH = 4.5 supplemented with 1 μg/ml of FK506. sRNA blots were hybridized with an antisense-specific probe to detect fkbA sRNA (see Methods). 5S rRNA probe served as a loading control. (B) Signals from blots presented in panel (A) were quantified by densitometry and the results were plotted. The bars indicate the standard deviation.