Table 3.
The effect of position 71 on RabMAb humanizations.
| H71 |
L71 |
|||||
|---|---|---|---|---|---|---|
| RabMAb | rabbit | humanized | rabbit | humanized | Affinity after humanization | Reference |
| YP3 | K | R | F | F | unchanged in protein and cell binding | |
| YP158 | K | R | Y | F | similar in protein and cell binding | |
| YP218 | K | R | Y | F | similar in protein and cell binding | 6 and the present study |
| YP223 | R | R | F | F | increased in cell binding and decreased in protein binding | |
| EBV321 | K | R | F | F | increased | 20 |
| anti-A33 | L | given the choice between L and R, resulting sequences have L/F = 4/2 | Y | given the choice between Y and F, resulting sequence have Y/F = 3/3 | clones selected based on high affinity | 4 |