Figure 11.

3D structure homology-modeling of wild type and mutated 6A and 8C bovine V genes. (A) 6A wild type and mutated mAbs. (B) 8C wild type and mutated mAbs. The 8C and 6A VH regions are shown in gray and the heavy CDRs are as follow: CDRH1: green, CDRH2: red and CDRH3: blue. The mutated residues are shown as black and with arrow in each mutated mAbs. the VH region was modeled using a fully automated protein structure homology-modeling server (SWISS-MODEL, http://swissmodel.expasy.org). The server used bovine ultra-long CDRH3 as template (Protein data bank code: 4k3d.1.A for 6A mAb and 4k3e.2.A for 8C mAb). The figures were generated using PyMOL program.⁸⁸