Table 1.

Effect of mutations on structural change and Env-binding.

Mutation	End-point concentration (ng/ml)	p value a	Structure change in 3D modeling	Mutation	End-point concentration (ng/ml)	p value a	Structure change in 3D modeling
8CWT	0.32			6AWT	8
8CY32A	1.6	***	−	6AW106A	1000	***	+
8CY59A	1.6	NS	−	6AC108A	1000<	***	+
8CW98A	1000	***	+	6AH111A	1000	***	+
8CC100A	200	***	+	6AY115A	40	***	+
8CC108A	200	*	NA
8CH109A	0.32	***	−

^a: p values were calculated using one-way ANOVA test to compare AUC of mutated antibodies graph compared with AUC of wild type antibody. Tukey HSD test was performed as post-test (

^*P<0.05,

^***P < 0.001, NS: Not significant). ng/ml: nanogram/milliliter, NA: Not applicable.