Examination of pluripotency of iPSCs derived neural progenitor cells. Neural progenitor cells were assessed by immunocytochemical analyses of the neural progenitor markers nestin (A,D,G, green), Pax6 (B,E,H, red) and Sox2 (C,F,I, red). Nuclei were counter stained with DAPI (blue). Scale show 100 µm. All pictures represent an overlay of fluorescence of immunocytochemical staining and DAPI staining.