Subject area	Cell biology
More specific subject area	Patient specific induced pluripotent stem cells and neuronal derivatives
Type of data	Phase contrast and fluorescence images, graphs of current and voltage traces of patch clamp recordings
How data was acquired	Fluorescence microscopy was performed with Keyence Biozero 8000 and a Carl Zeiss LSM780 microscope.
	Whole cell Patch clamp experiments in the current and voltage clamp mode were performed with a HEKA EPC-10-double amplifier with the according PatchMaster software
Data format	analyzed
Experimental factors	Human fibroblasts were transduced to induced pluripotent stem cells by retroviral transfection of Sox2, Klf4, Oct4 and c-Myc
Experimental features	Reprogramming of fibroblasts by retroviral system. Determination of karyotype by Giemsa Trypsin banding. Detection of marker for pluripotency by alkaline phosphatase (AP) staining and immunocytochemistry in induced pluripotent stem cells, embryoid bodies and neural progenitor cells. Specific marker for neurons and glial cells were detected by immunocytochemistry. Recording of functional ion channels by patch clamp technique.
Data source location	Albrecht-Kossel-Institute for Neuroregeneration (AKos), University Medicine Rostock, Rostock, Germany
Data accessibility	Data is provided with this article