Table 1.

PBD experimental result of the AAO pretreatment

Runs	X 1	X 2	X 3	X 4	Response (%)
					Cellulose retention	Hemicellulose retention	Delignification	Pretreatment yield
1	0.5	20	0.7	135	90.2	91.0	11.9	85.9
2	2.0	20	0.2	115	91.3	95.7	7.3	87.8
3	0.5	60	0.7	135	96.8	19.8	67.1	35.9
4	0.5	60	0.2	115	88.3	94.8	9.6	94.7
5	2.0	20	0.7	135	90.8	11.4	33.8	39.9
6	0.5	20	0.7	115	89.0	93.0	11.4	88.0
7	2.0	60	0.2	135	92.2	28.7	52.9	42.7
8	2.0	60	0.7	115	96.3	21.6	60.0	33.4
9	2.0	60	0.7	115	96.0	22.8	58.9	40.7
10	0.5	20	0.2	115	90.0	95.8	9.5	94.1
11	0.5	60	0.2	135	87.7	87.7	23.1	80.6
12	2.0	20	0.2	135	91.3	48.3	27.0	57.7

X ₁ time, X ₂ acetic acid concentrations, X ₃ H₂SO₄ addition, X ₄ pretreatment temperature