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. 2004 Dec;1(3):259–267. doi: 10.1093/ecam/neh043

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© 2004, the authors Evidenced-based Complementary and Alternative Medicine, Vol. 1, Issue 3 © Oxford University Press 2004; all rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated.

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ABH induces IL-12 gene expression. Ficoll-isolated human PBMCs were stimulated with 40 ng/ml ABH for the indicated times. RNase protection assay was performed with total RNA from stimulated PBMC. Aliquots of 10 μg of RNA were used for each determination. Lane 1, free probe; lane 2, yeast tRNA; lanes 3 and 4, unstimulated cells (–); lanes 5–8, cells stimulated with Agaricus blazei extract for the times indicated at the top of the panel. The upper panel shows IL-12p35 and p40 mRNA, and the lower panel shows L32 and GAPDH as a control. Data shown are representative of three independent experiments.