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Relative levels of Optn mRNA expression after anti-TCR(CD3)/CD28 stimulation of Jurkat T cells (A), D10 T cells (B) and CD3+ primary murine T cells (C), was assessed by real-time PCR. Results are shown as Optn Ct values normalized to the indicated endogenous control. Data are representative of three independent experiments. Statistical analysis was carried out on triplicate samples from a representative experiment, using the Kruskal-Wallis test. **p<0.01.
