Table 1.

Body weight, systolic BP, treadmill time, selected gene mRNA levels in cardiomyocytes

	Male		Female		P Values
	WT	KO	WT	KO	Sex	KO	Sex×KO
n	4	4	4	4
Body weight (g)	31.1 ± 0.8	28.0 ± 0.5	22.4 ± 0.9	22.4 ± 0.8	<0.0001	0.068	0.059
Systolic BP (mmHg)	108 ± 4	114 ± 3	109 ± 2	111 ± 2	0.813	0.223	0.544
Treadmill time (min)	18.8 ± 1.6	21.5 ± 1.6	33.6 ± 2.6	28.9 ± 1.9	<0.0001	0.624	0.117
Gene mRNA levels in cardiomyocytes
GPER	1.00 ± 0.08	0.44 ± 0.04	1.16 ± 0.26	0.39 ± 0.04	0.394	<0.001	0.081
ERα	1.00 ± 0.15	0.81 ± 0.07	0.87 ± 0.07	0.99 ± 0.24	0.869	0.814	0.320
ERβ	1.00 ± 0.14	0.81 ± 0.04	0.85 ± 0.12	0.79 ± 0.14	0.301	0.472	0.625
ANF	1.00 ± 0.27	3.38 ± 0.16	1.07 ± 0.18	3.73 ± 0.28	0.371	<0.0001	0.555
BNP	1.00 ± 0.11	2.25 ± 0.45	1.59 ± 0.33	2.97 ± 0.72	0.179	0.014	0.880

WT: wild type (GPER^f/f); KO: cardiomyocyte-specific GPER knockout (GPER^f/f/Cre); BP: blood pressure; ER: estrogen receptor; ANF: atrial natriuretic factor; BNP: brain natriuretic peptide. Two-way ANOVA was used to determine the significant differences in respect to sex (male vs. female), GPER status (WT vs. KO), and interaction (gender × GPER status).