Table 1.
Experimental conditions for optimization of cellulases production by T. reesei
| Experimental conditions for optimization of various parameters | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Fixed parameter | Name of parameter (under study) | ||||||||
| Incubation time (days) | Initial moisture level (solid-to-liquid ratio) | Temp (°C) | Inoculum size (no. of discs) | Inoculum age (h) | Carbon source | C-source concentration (%) | Nitrogen source | N-source concentration (%) | |
| Initial moisture level (solid-to-liquid ratio) | 1:2.5 | Observed | 1:2 | 1:2 | 1:2 | 1:2 | 1:2 | 1:2 | 1:2 |
| Temperature (°C) | 30 | 30 | Observed | 30 | 30 | 30 | 30 | 30 | 30 |
| Inoculum size (no. of discs) | 1 | 1 | 1 | Observed | 2 | 2 | 2 | 2 | 2 |
| Inoculum age (h) | 120 | 120 | 120 | 120 | Observed | 96 | 96 | 96 | 96 |
| Carbon source | – | – | – | – | – | Observed | Lac | Lac | Lac |
| Carbon source concentration (%) | – | – | – | – | – | 1 | Observed | 1.5 | 1.5 |
| Nitrogen source | – | – | – | – | – | – | – | Observed | AmmMol |
| Nitrogen source concentration (%) | – | – | – | – | – | – | – | 1 | Observed |
| Optimized parameter for CMCase | 8 | 1:2 | 30 | 2 | 96 | Lac | 1.5 | AmmMol | 1 |
| Optimized parameter for FPase | 8 | 1:2 | 30 | 2 | 120 | Lac | 1-1.25 | AmmMol | 1 |
| CMCase activity (U/gds) | 10.66 ± 0.29 | 11.65 ± 0.38 | 11.71 ± 0.23 | 14.34 ± 0.26 | 15.95 ± 0.33 | 17.21 ± 0.17 | 18.23 ± 0.34 | 20.41 ± 0.14 | 20.49 ± 0.20 |
| FPase activity (U/gds) | 1.19 ± 0.28 | 1.29 ± 0.17 | 1.31 ± 0.34 | 1.65 ± 0.20 | 1.79 ± 0.05 | 1.99 ± 0.28 | 2.10 ± 0.30 | 2.40 ± 0.09 | 2.42 ± 0.08 |
Lac lactose, AmmMol ammonium molybdate