Table 2.
mRNA expression of PPARs-responsive genes in PPAR-transfected MCF-7 cells (with overexpression of PPARs) after treatment with experimental FA or specific agonist/antagonist of PPAR for 48 h.
| Gene symbol | FC values ± SD | |||||
|---|---|---|---|---|---|---|
| EFA versus NC | EFA-CLA versus NC | cis9,trans11CLA versus NC | trans10,cis12CLA versus NC | Agonist versus NC | Antagonist versus NC | |
| (A) mRNA expression of PPARα-dependent genes | ||||||
| TCF-20 | −1.00∗ ± 0.02 | 1.02 ± 0.03 | 1.10 ± 0.24 | 1.70∗ ± 0.14 | 1.24 ± 0.14 | −3.09∗ ± 0.23 |
| WT1 | −1.32 ± 0.11 | −1.49∗ ± 0.11 | −1,86∗ ± 0.07 | −1.31 ± 0.23 | 1.34 ± 0.26 | −2.11 ± 0.18 |
| ZNF621 | 1.36 ± 0.16 | 1.80∗ ± 0.20 | −2.51∗ ± 0.04 | 1.09 ± 0.19 | −1.06 ± 0.18 | −1.20 ± 0.24 |
| THRB (TRβ2) | 2.49 ± 0.08 | 1.15 ± 0.12 | 1.74∗ ± 0.09 | 7.98∗ ± 0.34 | 2.54 ± 0.22 | −1.77∗ ± 0.00 |
|
| ||||||
| (B) mRNA expression of PPARδ-dependent genes | ||||||
| TCF-20 | 2.03∗ ± 0.04 | 3.08∗ ± 0.03 | 7.05∗ ± 0.11 | 13.02∗ ± 0.08 | −1.43∗ ± 0.01 | 2.36 ± 0.08 |
| WT1 | 1.18 ± 0.26 | −1.38∗ ± 0.03 | −1.52∗ ± 0.05 | 1.71 ± 0.29 | 1.90∗ ± 0.04 | 1.81∗ ± 0.01 |
| ZNF621 | 1.37∗ ± 0.03 | −1.29∗ ± 0.02 | 1.09 ± 0.16 | −1.26 ± 0.19 | −1.76 ± 0.11 | −1.23∗ ± 0.03 |
| THRB (TRβ2) | 1.61∗ ± 0.02 | 1.33∗ ± 0.01 | 6.67∗ ± 0.09 | 18.15∗ ± 0.11 | 1.90∗ ± 0.04 | 1.81∗ ± 0.01 |
|
| ||||||
| (C) mRNA expression of PPARγ-dependent genes | ||||||
| TCF-20 | 2.09∗ ± 0.03 | 3.21∗ ± 0.04 | 6.66∗ ± 0.16 | 13.48∗ ± 0.09 | 1.92∗ ± 0.03 | 2.00∗ ± 0.01 |
| WT1 | −1.02 ± 0.04 | −1.24 ± 0.06 | −1.32∗ ± 0.03 | −1.02 ± 0.08 | 1.48 ± 0.07 | −1.47∗ ± 0.03 |
| ZNF621 | 2.99∗ ± 0.01 | 3.12∗ ± 0.17 | −1.13 ± 0.05 | 1.46 ± 0.07 | 5.97∗ ± 0.20 | 3.76∗ ± 0.10 |
| THRB (TRβ2) | 1.09 ± 0.01 | 1.14 ± 0.01 | 9.96∗ ± 0.10 | 17.22∗ ± 0.13 | −1.91∗ ± 0.01 | −1.58∗ ± 0.00 |
FC: fold change; NC: negative control. Agonist/antagonist: for PPARα, WY14643/GW-6471; for PPARδ, GW-0742/GSK0660; for PPARγ, troglitazone/T0070907. ∗p < 0.05.