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. 2017 Mar 26;2017:5318232. doi: 10.1155/2017/5318232

Table 1.

S. cerevisiae strains and plasmids used in this work.

Genotype/properties Source/reference
Strain
BSW3AP CEN.PK102-3A derivative, gre3(−241, +338):: TPI1p–RKI1–RKI1t–PGK1p–TAL1–TAL1t–FBA1p–TKL1–TKL1t–ADH1p–RPE1–RPE1t–loxP, {YIp5-ara, pYX2422-TEF1araA}, selected for growth on L-arabinose [29]
BSW4AP BSW3AP derivative, discarding plasmid pYX2422-TEF1araA Present work
BSW4XA1 BSW4AP derivative, {pYX242-XIA} Present work
BSW4XA2 BSW4XA1 derivative, {pYX242-XIA, pYMIK-xy127} Present work
BSW4XA3 BSW4XA2 derivative, selected for growth on D-xylose Present work
Plasmid
pYX2422-TEF1araA pYX242-PGK1t-TEF1p-araA [29]
pYX242-XIA pYX242-Ru-xylA-PGK1t-TEF1p-araA Present work
pYMIK-xy127 Integration plasmid, KanMX4, ADH1p-XYL1-ADH1t, PGK1p-XYL2-PGK1t, PGK1p-XKS1-PGK1t [32]