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The main DNA primers used in this work.
| Primers | Sequence (5′-3′) | Purpose |
|---|---|---|
| One-XI up | GCTTAAATCTATAACTACAAAAAACACATACAGGAATTCATGGCAAAAGAATATTTTCC | Cloning Ru-xylA genes |
| One-XI down | TAGAGACATGGGAGATCCTAGCTAGCTAGATCCATGGTTATTTGCAGTGGAGGGCGACG | |
|
| ||
| pYX242-ce-F | GGAGTTTAGTGAACTTGCAAC | To validate or sequence the plasmid pYX242 |
| pYX242-ce-R | CGACTCACTATAGGGCGAATTG | |
| PGKt-pYX2422-R | ATACGCTGAACCCGAACATAG | |
