FIG. 1.

Characterization of cell line permissivity for Vif-defective HIV-2. (A) The abilities of HIV-2_ROD and HIV-2_RODSTOP viruses to replicate were compared in several cell lines, namely, SupT1, A3.01, U38, and HeLaCD4⁺, Jurkat, and H9 cells and PBMC. HIV-1_NL43 and HIV-1_NL43 vif-minus viruses were used for control of cell line permissivity character. Quantification of p26 levels was performed in cell culture supernatants 10 days after infection. PBMC are the only cells corresponding to the nonpermissive character for HIV-2 replication in the absence of Vif. (B) Northern analysis of APOBEC3G expression in A3.01, Jurkat, H9 HeLa CD4⁺, SupT1, and U38 cells. RNAs extracted from uninfected cells were resolved by electrophoresis and subjected to Northern analysis with ³²P-labeled APOBEC3G probe (top panel). For internal control, a β-actin specific probe was used (bottom panel).