Fig. 5.

Aged mice exhibit locomotor deficits. (A) Wild-type C57BL/6 mice were tested on a standard rotarod apparatus for two days with 4 trials per day at the ages of 3 months (n=14), 18 months (n=14), 24 months (n=14) and 30 months (n=13). (B and C) The mice were tested in the open field assay and distance traveled and center:total distance ratio were analyzed. Note, advanced aged mice (30 month old mice, Black; n=13) travel less total distance compared to 3 month old (white; n=14) and 18 month old (red; n=14) mice (*, p<0.05). Data represent the distance traveled (B) and the center:total distance ratio (C). (D) Mice underwent elevated plus maze testing for anxiety-like behavior. Data represent the ratio of time spent in the open arms vs. the closed arms of the elevated maze. Data are plotted as mean ± SEM. *p<0.05.