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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Neuron. 2016 Jun 30;91(2):356–369. doi: 10.1016/j.neuron.2016.06.013

Figure 6. SRGAP2A regulates the assembly of excitatory and inhibitory postsynaptic scaffolds.

Figure 6

(A–B) Representative Western blots (A) and quantifications

(B) of the synaptic abundance of the indicated proteins in P15 wild-type (WT) and Srgap2a KO brains (n=4).

(C) Immunofluorescence of endogenous proteins in cortical neurons cultured for 17–18 days in control condition (shControl) or after Srgap2a knock-down (shSrgap2). Scale bar 2 μm.

(D) Normalized fluorescence intensity (Norm. Fluo.) associated with clusters of the indicated proteins in shControl and shSrgap2 conditions (n=40–42 cells).

(E) Live imaging of SEP-GluA2 and SEP-γ2 GABAA receptors in control pyramidal neurons, after Srgap2a knock-kown or after SRGAP2C expression (20–22 days in vitro). Scale bar: 2 μm.

(F) Mean receptor cluster size in the conditions described above. SEP-GluA2: nshControl = 34, nshSrgap2 = 35, nSRGAP2C = 32 ; SEP-γ2: nshControl = 36, nshSrgap2 = 25, nSRGAP2C = 35. Mean ± SEM.

(G) Representative traces of mEPSCs in control (black) and shSrgap2-electroporated (red) neurons.

(H–I) Quantification of mEPSC amplitude (nControl = 2883 from 5 cells, nshSrgap2 = 2811 from 5 cells) and interevent intervals (nControl = 7232, nshSrgap2 = 6587). Cumul. Prob.: cumulative probability. Inset indicates the mean frequency per cell.

(J) Representative traces of mIPSCs in control (black) and shSrgap2-electroporated (red) neurons.

(K–L) Quantification of mIPSC amplitude (nControl = 4370 from 7 cells, nshSrgap2 = 4125 from 7 cells) and interevent intervals (nControl = 11520, nshSrgap2 = 8262). Inset indicates the mean frequency per cell.

*** p < 0.001, NS: p > 0.05, Mann-Whitney test or Kruskal-Wallis test followed by Dunn’s multiple comparison test (panel F).