Figure 3. Effect of ice plant extract on the expression of iNOS mRNA in IFN-γ-stimulated macrophages. (A) Cells were treated with IPE (5 µL/mL) and co-treated with IFN-γ (100 ng/mL) for the indicated time. (B) Cells were treated with the indicated concentration of IPE and co-treated with IFN-γ (100 ng/mL) for 6 h. iNOS gene expression was analyzed using real-time qPCR and results were normalized to the expression of GAPDH. (C) Cells were treated with various concentrations of IPE for 24 h. The nitrite content in the medium was determined by Griess reagent. Data are shown as the mean±SD of triplicate samples from one representative experiment of three independent experiments. ^**P< 0.01 vs control, ^***P< 0.001 vs control.