FIG. 5.

Incorporation of endocytosed gB into VZV virions. Virions were isolated as described in the legend to Fig. 3 (lanes 7 and 9). Cell lysates were collected before the isolation of virions (lanes 6 and 8). Immunoprecipitated gB samples (lanes 1 to 7) or samples without immunoprecipitation (lanes 8 and 9) were separated by SDS-8% PAGE under nonreducing conditions (lanes 1 to 7) or native conditions (lanes 8 and 9). Biotinylated glycoproteins were detected with Streptavidin-HRP (lanes 1 to 7). In lanes 8 and 9, Western blotting was performed with an anti-gB MAb. Lanes 1 to 4, lanes 5 to 7, and lanes 8 and 9 represent three separate gels. The corresponding molecular mass markers are indicated on the left.