Table 2.

Kinetic parameters.

		KM	VM	kcat	kcat/KM
GAPDH	Substrate	(mM)	(mM s−1 mg−1)	(s−1)	(mM−1 s−1)
A. vaginae	G3P	2.6 ± 0.5	5.7 ± 0.3	57 ± 6	22 ± 5
	NAD+	0.08 ± 0.03	3.1 ± 0.2	31 ± 3	384 ± 150
	Pi	3.4 ± 0.6	2.9 ± 0.2	29 ± 3	8 ± 2
S. aureusa	G3P	0.32	n.d.	70	218.8
	NAD+	0.316	n.d.	n.d.	n.d.
	Pi	2.57	n.d.	n.d.	n.d.

^aThe kinetic parameters of SaGAPDH were taken from Mukherjee et al. (2010).