Figure 5.

Defining the metazoan leptin system. (A) UPD2 model (gray) aligned with human LEP (hLEP, red). To the right of the structure overlay are identified amino acids in red that are conserved in the two proteins. Below the models are sequence alignments showing amino acids conserved in the top two motifs (gray), conserved posttranslational modifications (PTMs) (red), and known sites to interact with the receptor (cyan). (B) Dome model with amino acids in red conserved with human leptin receptor (LEPR). To the right of the model are sequence alignments showing amino acids conserved in the top seven motifs (gray) and conserved PTMs (red). (C) Using the top motifs of LEP, LEPR, and LEPROT, BLAST data for each in invertebrate genomes. (D,E) Models of LEPROT, now known as endospanin 1 (D), with conserved amino acids identified (E). Amino acids in red are conserved in all sequences, those in green conserved in at least four of the five groups, those in cyan conserved in at least three groups, and those in gray conserved in at least two groups.