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. 2017 Apr 10;8:615. doi: 10.3389/fmicb.2017.00615

FIGURE 1.

FIGURE 1

Effect of environmental cues on expression of transcription factors. (A) Expression (qRT-PCR) of the transcription factors of H. pylori 26695 in exponential (black bars) and stationary growth phase (gray bars). (B) Determination of colony forming units (CFU) of H. pylori 26695 grown to stationary phase (48 h) in BB + FBS under acid pH (pH 5.5), or in presence of urea (5 mM), nickel (250 μM), or iron (150 μM). Fold-change expression (qRT-PCR) of the transcription factors under acid pH (C), urea (D), nickel (E), and iron (F). Data is represented as fold-change expression of the regulatory gene under different environmental conditions as compared to plain BB + FBS at 48 h. Data represent means and standard deviations of at least three independent experiments. ns, not significant; statistically significant ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05.