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. 2017 Apr 10;8:67. doi: 10.3389/fendo.2017.00067

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Copyright © 2017 Amri, Ghouili, Tonon, Amri and Masmoudi-Kouki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Hb on H₂O₂-induced ROS and NO intracellular accumulations. Cells were co-incubated for 24 h with medium alone () or with H₂O₂ (50 µM) in the absence () or presence of graded concentration of Hb (10⁻¹² to 10⁻⁶ M, ). (A) Cellular ROS level was quantified by measurement of DCF fluorescence intensity. (B) Cellular NO level was quantified by measurement of DAF fluorescence intensity. Inset, effect of graded concentration of Hb (10⁻¹² to 10⁻⁶ M; ) on intracellular ROS accumulation (A) and NO formation (B). The results are expressed as percentages of the controls. Data are means ±SEM of three independent experiments. ANOVA followed by Bonferroni’s test *p < 0.05; **p < 0.01; ***p < 0.001; NS, not statistically different from control cells. ^#p < 0.05; ^##p < 0.01; ^###p < 0.001; ns, not statistically different vs. H₂O₂-treated cells.

Inline graphic — Effect of Hb on H₂O₂-induced ROS and NO intracellular accumulations. Cells were co-incubated for 24 h with medium alone () or with H₂O₂ (50 µM) in the absence () or presence of graded concentration of Hb (10⁻¹² to 10⁻⁶ M, ). (A) Cellular ROS level was quantified by measurement of DCF fluorescence intensity. (B) Cellular NO level was quantified by measurement of DAF fluorescence intensity. Inset, effect of graded concentration of Hb (10⁻¹² to 10⁻⁶ M; ) on intracellular ROS accumulation (A) and NO formation (B). The results are expressed as percentages of the controls. Data are means ±SEM of three independent experiments. ANOVA followed by Bonferroni’s test *p < 0.05; **p < 0.01; ***p < 0.001; NS, not statistically different from control cells. ^#p < 0.05; ^##p < 0.01; ^###p < 0.001; ns, not statistically different vs. H₂O₂-treated cells.