Figure 1.

Schematic representation of soybean barcode system using InDel markers. (A) Sequence diversity generation by recombination events during the cross-breeding process. The rectangle represents the sequences, and the different colors indicate the divergence in the sequences. (B) Two steps were used for developing barcode system. In step 1, two types of blocks, the dense variation blocks (dVBs, gray) and the sparse variation blocks (sVBs, white), were determined by the sequence comparisons of cultivar genomes. In step 2, selection of dVB-specific InDels and bar-coding process. At each chromosomal position, dVBs of identical types were represented by the same color compared to reference genome. Based on the results of dVB-specific InDel marker amplification, the same results with the reference genome (RG) were represented by “a” (white), the other results by “b” (black).