Table 1.

Primers used for the exploration of differentially methylated CpG sites in the canine OXTR promoter.

Primer Name	Sequence	Genomic coordinates (Chromosome 20)	Type	Quality score
P1_F	5′ TGA TGT AAT TTT TAA GGG TAA GAA AAA GAT A 3′	9357389 : 9357419	Amp	–
P1_R	5′ TTT AAA TAC ATT CTT CCT CCT AAC ATT TCC TTT C 3′	9357608 : 9357641	Amp	–
P1_S1	5′ AAT TTT TAA TTT TTT TTA ATG TTG T 3′	9357419 : 9357442	Seq	74
P1_S2	5′ TTA ATT AGA ATT TTG GGA TT 3′	9357476 : 9357495	Seq	76
P1_S3	5′ GGT ATA GGG TTG TAA TTG 3′	9357530 : 9357547	Seq	79
P2_F1	5′ AGG GTG ATG AAG TTG TAA AAG T 3′	9358139 : 9358160	Amp	–
P2_F2	5′ AGG GAA AGA TTT TAA GAA AAG ATA AGA AAG 3′	9357913 : 9357938	Amp	–
P2_R	5′ ACA TTT CAT CTT CCT TTA ACA TCA TAT A 3′	9357788 : 9357815	Amp	–
P2_S1	5′ ATG AAG TTG TAA AAGTAT TTA ATT G 3′	9358130 : 9358154	Seq	71∗
P2_S2	5′ TAA GTA AAT GTT TGT TTT GGA GTA 3′	9358026 : 9358049	Seq	68∗
P2_S3	5′ AAT TTA TTT TTA TTT TAA AGT GAT T 3′	9357875 : 9357899	Seq	80#
P3_F	5′ GG TTT TTG GAT GGG GAT AGG A 3′	9358485 : 9358505	Amp	–
P3_R	5′ ACT TCA TCA CCC TCT TCT CA 3′	9358148 : 9358167	Amp	–
P3_S1	5′ TTT TTG GAT GGG GAT AGG 3′	9358486 : 9358503	Seq	68
P3_S2	5′ GGT AGG AGG TAA AAA AAA G 3′	9358450 : 9358468	Seq	68
P3_S3	5′ GTT GGG GAG AGT TTT TTT GTA GT 3′	9358416 : 9358438	Seq	69
P3_S4	5′ GTA TAG TTT TAA GGG GTT ATT GGG 3′	9358378 : 9358401	Seq	72
P3_S5	5′ ATT TTT AGA TTA GGG TTA GTT TGG A 3′	9358330 : 9358354	Seq	72
P3_S6	5′ AAT TAG TAG TTT TAT TTT ATT TAA G 3′	9358288 : 9358312	Seq	69
P3_S7	5′ GGT TTT TTT TTT TTT TGG TTT AGA A 3′	9358217 : 9358241	Seq	63

Genomic coordinates are according to CanFam3.1 (GCA_000002285.2). Quality scores (<40: poor; 40–59: low; 60–87: medium; > 88: high quality) are assigned as by the PyroMark Assay Design Software and refer to primer sets (including forward, reverse and sequencing). Amp: amplifying; Seq: sequencing. ^∗For amplification, P2_F1 forward primer was used. ^#For amplification, P2_F2 forward primer was used.