TABLE 2.

Pseudotyped HIV-1_JR2 and MPER Ala mutants in a single-round infection neutralization assay with U87.CD4.CCR5 cells against antibodies b12, 2F5, and 4E10 and peptide T20

^aInfectivity of Ala mutant pseudoviruses relative to parental HIV-1_JR2 as measured by relative light units (RLU) produced in target cells by viral supernatants. +++, infectivity similar to that of wild-type HIV-1_JR2; ++, >30% of wild-type infectivity; +, <30% of wild-type infectivity; (+), <10% of wild-type infectivity.

^bConcentrations are in micrograms per milliliter. IC₅₀ and IC₉₀ values that are >3-fold that of the parental (JR2) virus are highlighted in pink (mutant virus more neutralization resistant), whereas values that are less than one-third that of the IC₅₀ or IC₉₀ of JR2 are highlighted in gold (mutant virus more neutralization sensitive). Values represent the averages of three independent experiments performed in triplicate.

^cValues preceded by ≈ are approximate. Neutralization curves were shallow, so that there was greater error at the 50% infectivity level (see text).