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. 2017 Apr 7;23(13):2330–2336. doi: 10.3748/wjg.v23.i13.2330

Table 1.

Sequences of primers for quantitative real-time reverse transcription polymerase chain reaction

Gene Forward Reverse
Notch1 5’-TCGTGCTCCTGTTCTTTGTG-3’ 5’-TTCTCTCCGCTTCTTCTTGC-3’
Notch2 5’-TGATGAGCAGGAACAGGAGA-3’ 5’-ATGAGAAGCCAGGAGAGCAG-3’
Notch3 5’-ATACCCACTACGGGATGTGC-3’ 5’-AGGACGAAGATGACCAGCAG-3’
Notch4 5’-GGATGAATGTCGGAGTGACC-3’ 5’-GGCTACACAAGGGAACCTCA-3’
Jagged1 5’-CAGTGGCTTGGGTCTGTTG-3’ 5’-CATTGTTGGTGGTGTTGTCC-3’
Hes1 5’-GTGGGTCCTAACGCAGTGTC-3’ 5’-GTCAGAAGAGAGAGGTGGGCTA-3’
Hes5 5’-ATGCTCAGTCCCAAGGAGAA-3’ 5’-CTCCAGCAGCAGTTTCAGC-3’
TGF-β1 5’-ATTCCTGGCGTTACCTTGG-3’ 5’-AGCCCTGTATTCCGTTCTCT-3’
Smad2 5’-CGGCTGAACTGTCTCCTACC-3’ 5’-AGGTCTCTCCAACCCTCTGG-3’
Smad3 5’-GGTAAAGGATTGCCACCAAA-3’ 5’-GAACAGCCAGGAAAGGGACT-3’
β-actin 5’-CACCCGCGAGTACAACCTTC-3’ 5’-CCCATACCCACCATCACACC-3’