FIG. 4.
EBV gp42 associates with HLA-DR molecules intracellularly shortly after biosynthesis. (A) MJS/gp42 cells were metabolically labeled with [35S]Cys (lanes 1 to 6) or with [35S]Met (lanes 7 to 18) for 30 min and chased for the times indicated. For immunoprecipitation (IP), rabbit antiserum #32 against gp42 and MAb Tü36 against HLA-DR were used. (B) Half of the immunoprecipitates were treated with PNGase F to remove N-linked glycans. Immune complexes were denatured in nonreducing (A) or reducing (B) sample buffer and analyzed by SDS-12% PAGE.