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. 2017 Apr 1;33(3):149–160. doi: 10.1089/jop.2016.0093

FIG. 2.

FIG. 2.

The detection of apoptosis in retinal sections of each group of rats. (A) TUNEL staining (green) of retinal sections (site b) of the eyes with intravitreous injection of 3 μL saline in the right eye or series of dilutions of the dye stock solution in the left eye under 12-h light–dark cycle. Eyes were enucleated 2 weeks after the first injection at the age of 4 weeks. The nuclei were counterstained with DAPI (blue). The number of TUNEL-positive cells in the outer nuclear layer (ONL) was less in the dye-injected eyes than in the saline-injected eyes. (B) TUNEL staining of retinal sections at 4 different retinal sites (a, b, c, and d) in the left eye with dye injection (16 μM) compared with that in the right eye with saline injection under 12-h light–dark cycle. (C) TUNEL staining of retinal sections at 4 different retinal sites (a, b, c, and d) in the left eye with dye injection (16 μM) compared with that in the right eye with saline injection under 24-h constant dark condition. INL, inner nuclear layer; OPL, outer plexiform layer. Scale bar: 10 μm. DAPI, 4′,6-diamidino-2-phenylindole.