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. 2017 Apr 1;33(3):149–160. doi: 10.1089/jop.2016.0093

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© Shihui Liu, et al., 2017; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 5. — Quantitative analysis of the thickness of the ONL of each group of rats. The thickness of the ONL of 4 different retinal sites (a, b, c, and d) of the left eye with dye injection at 5 concentrations compared with that of the right eye with saline injection under 12-h light–dark cycle. The thickness showed significant differences among different concentrations of the dye (P = 0.0001) and among 4 different retinal sites (P = 0.014, 2-factor ANOVA). The ONL was significantly thicker with dye injection at 3 concentrations, 16 μM (**P = 0.001), 16 μM × 0.1 (**P = 0.001), and 16 μM × 0.01 (*P = 0.026), than with saline injection. The bottom right panel shows the ONL thickness of the eyes injected with dye (16 μM) versus saline under 12-h light–dark cycle versus under 24-h constant dark condition. There was no significant difference between dye-injected eyes and saline-injected eyes under 24-h dark condition. T bars indicate standard deviation.