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Temperature sensitivity of ts53 virus in plaque formation. Plaque assays were carried out at 34°C (permissive temperature) and 39.5°C (nonpermissive temperature) with diluted (103 to 106 times) wild-type WSN/33 virus and ts53 virus. The efficiency of plaque formation of WSN/33 virus at 39.5°C relative to that at 34°C (EOP39.5/34) was 0.1, whereas the EOP39.5/34 of ts53 virus was 1.0 × 10−4.
