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Analysis of the time to reach deep anesthesia within different zebrafish lines using MS-222. The groups of zebrafish tested were wild type (AB), Transgenic [Tg (KDRmCherry:cmlc2GFP)], Casper (roy−/roy−; nacre−/nacre−), TraNac (tra−/tra−; nacre−/nacre−), and “TraNac Transgenic” [TraNac Tg (KDR:mCherry:Fabp10-rtTA:TRE-eGFPKRASV12)]. Within each group, n = 5 and the error bars represent the SEM. *p < 0.05 and N.S. indicates not significant. SEM, standard error of the mean.
