FIG. 3.

Amino acid alignment of α1 and α2 domains of 20 novel pigtail macaque MHC class I alleles. Up to 96 MHC class I cDNA clones from six pigtail macaques underwent forward and reverse sequencing, generating 700- to 1,000-bp fragments. Alleles were defined where at least three identical clones with full contiguous sequence were detected (Mane-B*22 was defined due to its close relationship to Mane-B*11 and Mane-B*12 despite only being present in two clones). Predicted amino acid translations of the α1 and α2 domains, the peptide binding regions, are shown. The leader, α3, transmembrane, and cytoplasmic sequences were also identical within each defined allele (data not shown; see GenBank accession numbers AY557348 to AY557367). Closely related alleles are boxed together (see the text and Table 2). Boldface alleles represent newly described alleles. Alleles not in boldface were previously described (38) and are provided for comparison. We confirmed the presence of Mane-B*02 and Mane-B*05 in pigtail macaques studied in this report (marked with a superscript letter b), with the additional clones and numbers of animals consolidated.