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. 2017 Apr 10;7:46064. doi: 10.1038/srep46064

Figure 6. Mincle-mediated degranulation in RBL-2H3 cells.

Figure 6

RBL-2H3 cells and cells expressing WT Mincle or the R42I mutant were stimulated with or without 10 μg/ml anti-myc mAb for 30 min (anti-myc) or preincubated overnight with anti-DNP IgE mAb and then stimulated with 30 ng/ml DNP-BSA for 30 min (DNP). The amount of β-hexosaminidase released from these cells was determined. Data are representative of more than three independent experiments using PA-11 (WT) and R42I-3 (R42I) cell lines and are presented as the mean ± S.D. (*P < 0.001 was considered significant. n = 3/group). Similar results were obtained from the other cloned cell lines.