FIG. 1.

Genome organization of potato leafroll virus (PLRV) and description of the CP mutants. The wild-type amino acid sequence of the two domains of the acidic patch are provided in the single-letter code. The domains are separated by 55 amino acids. Bold letters indicate residues that are conserved among all poleroviruses whose sequence is known. The five acidic amino acids (D or E) are in italics. The 11 single and multiple amino acid mutants are defined under the wild-type sequence. The amino acid changes (all alanine substitutions except one) are underlined and are shown in the center of the three- or four-letter mutant designations. The numbers indicate the amino acid residue position within the PLRV CP. Note that the upstream domain overlaps the virus movement protein (P17) encoded by ORF 4, whereas the downstream domain does not.