TABLE 1.
Virus antigen titer in tissue infiltrated with wild-type or acidic patch mutants of PLRV
| PLRV | Virus antigen in tissuea (% of wild-type level)
|
|
|---|---|---|
| DAS-ELISA | TAS-ELISA | |
| Wild type | 100 | 100 |
| HeY | 15-50 | 40-100 |
| GvE | 100 | 100 |
| VeW | 0 | 0 |
| EwH | 0 | 0 |
| WhD | 0 | 0 |
| HdS | 5-20 | 40-100 |
| DssE | 100 | 100 |
| SeD | 10-50 | 50-100 |
| Sed | 0 | 0 |
| EdQ | 0 | 0 |
| CPall | 0 | 0 |
a
Range of virus antigen levels in tissue agroinfitrated with the various mutants determined in two or three independent experiments. A value of zero indicates that absorbance values were not above the background (<0.05). Double-antibody sandwich ELISA used the same PLRV-specific polyclonal antibodies for trapping and detection. The detection antibody was conjugated to alkaline phosphatase. The triple-antibody sandwich ELISA trapped virus with a PLRV-specific polyclonal antibody and detected it with a PLRV-specific monoclonal antibody.