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Subcellular distribution of HCV core protein analyzed by immunoblotting. ER and mitochondrial (Mt) fractions were isolated from 293T cells expressing the full-length core protein (Core) or nontransfected cells (Mock) 2 days after transfection. Equal amounts of protein from each fraction as well as whole cell lysates (Total) were subjected to immunoblotting with a monoclonal antibody against either HCV core, prohibitin, or ribophorin I.
