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. 2017 Apr 10;7:45458. doi: 10.1038/srep45458

Figure 3. Validation of the new NikR-dependent nickel-regulated ncRNAs.

Figure 3

Top panels: transcriptional analysis of ncRNAs in wt/ni−, wt/ni+, ∆nikR/ni− and ∆nikR/ni+ conditions. (A) Northern blot of Nrr1 (left) and quantitative RT-qPCR of its transcript levels (right) (see legend 2D for details). (B) Primer extension analysis of the Nrr2 transcript. (C) Northern blot of the IsoB transcript. Middle panels: DNase I footprinting of radiolabeled Nrr1 (A), Nrr2 (B) and IsoB (C) DNA probes, mixed with 0, 9.7 (only panel B), 29, 97 and 290 nM of the NikR tetramer, without nickel (left side of each panel) or with the addition of 150 μM NiSO4 (right side of each panel). Uncropped blots and gels are provided in the Supplementary Information. Legends and symbols as in Fig. 2.