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. 2017 Apr 10;7:46054. doi: 10.1038/srep46054

Figure 13. Interaction of ClVelB with ClVeA and ClVosA in C. lunata.

Figure 13

(A) ClVelB interacts with ClVeA and ClVosA in a Y2H approach. For Y2H analysis, the clvelb cDNA was fused to the GAL4 activation domain, and the cDNAs of clveA and clvosA were respectively fused to the GAL4 binding domain. The cell suspensions of the Saccharomyces cerevisiae strain (AH109) containing the indicated vectors were dropped onto the selected media. SD-LT: synthetically defined (SD) medium without leucine and tryptophan was used to demonstrate the presence of both vectors. SD-LTHA: SD without leucine, tryptophan, histidine, and adenine supplemented with 11 mM 3-amino-1,2,4-triazole (SD_LWH+3-AT) was used to detect HIS3 reporter gene activity (displayed by colony growth). Plasmid pairs of pGADT7-SV40/pGBKT7-53 and pGADT7-SV40/pGBKT7-Lam served as the positive and negative controls, respectively. (B) Interaction analysis of ClVelB with ClVeA and ClVosA versions by BiFC assay in infiltrated Nicotiana benthamiana leaves. Co-expression of ClVelB with ClVeA and ClVosA versions fused to splitYFP. n/cYFP: n- or c-terminal part of splitYFP.