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. 2017 Apr 10;7:46054. doi: 10.1038/srep46054

Figure 9. ClVelB regulates cell wall integrity.

Figure 9

(A) Sensitivity of the WT strain (CX-3), clvelB deletion mutant (ΔClVelB), and complemented strain (ClVelB-C) to the cell wall damaging agents. The detection was made on a CM plate added with the corresponding cell wall damaging agent. (B) Expression changes of gls2 and slt2 in each strain. The relative expression levels of gls2 and slt2 in ΔClVelB are the relative cDNA amounts of the same gene in the WT strain. Line bars indicate the standard errors from the three trial replicates. A single asterisk indicates a p-value < 0.05 while double asterisks indicate a p-value < 0.001 in the T-test analysis. (C) Ultrastructural analyses of the cell of the clvelB deletion mutant. Cells of the WT and ΔClVelB were observed with a transmission electronic microscope (Tecnai G2 Spirit Biotwin, FEI). Mycelia were harvested and fixed in glutaraldehyde for 12 h at 4 °C.